Haryana Vet. 49 (December, 2010), pp 66-67
Research Article
D. MITTAL1, G. FILIA2, Y. SINGH and J. SINGH Regional Research and Training Centre, Guru Angad Dev Veterinary and Animal Science University 2Department of Epidemiology and Preventive Veterinary Medicine, College of Veterinary Sciences Guru Angad Dev Veterinary and Animal Science University, Ludhiana ABSTRACT
Caseous lymphadenitis caused by Corynebacterium pseudotuberculosis is a common disease of small ruminants. Twelve cases of this disease were recorded at an organized goat farm that had a total strength of 70 animals. C. pseudotuberculosis was isolated fromthese cases which was confirmed on the basis of biochemical and sugar fermentation tests. Antimicrobial sensitivity pattern revealed thatthe organisms were sensitive to ciprofloxacin, cephalexin, enrofloxacin, oxytetracycline, cotrimoxazole, cephalothin and tetracycline.
Resistance to streptomycin, norfloxacin, oxacillin and nitrofurantion was observed while chloramphenicol and ampicillin gave theintermediate sensitivity. The affected animals were treated with antimicrobials and supportive therapy in recommended doses. The pusmaterial from the abscess area was drained with the care that the material did not soil the bedding or floor of the farm.
Key words: Caseous lymphadenitis, Beetal goats
plates after inoculation were incubated at 370C for 24- Corynebacterium pseudotuberculosis, is a chronic 48 h. Identification and confirmation of the organism contagious disease affecting mainly sheep and goats.
was done on the basis of morphological, biochemical The disease is worldwide in nature and is transmitted and sugar fermentation tests. The organisms were mainly through contamination of superficial wounds.
Gram stained and observed to study Gram's reaction, The ability of the organism to survive in the animal's shape and arrangement. The biochemical tests included environment for several weeks contributes to its ability nitrate reduction, catalase and urease. Sugar to spread within a flock. Economic losses result mostly fermentation reaction was also carried out with glucose, from condemnation of infected carcasses and devaluation of hides. The objective of this study was Antimicrobial sensitivity pattern: Antimicrobial
to report this disease at an organized Beetal goat farm sensitivity pattern of the organisms was determined by in Bathinda district of Punjab state.
modified Kirby-Bauer method (Carter, 1973) on MuellerHinton Agar (MHA) plates. The antimicrobial discs (Hi MATERIALS AND METHODS
Media) used were chloramphenicol (30 mcg), The present study was carried out at an organized enrofloxacin (10 mcg), oxytetracycline (30 mcg), Beetal goat farm from January to December, 2010. At cotrimoxazole (25 mcg), ciprofloxacin (10 mcg), this farm, semi-intensive management system was streptomycin (10 mcg), cephalexin (30 mcg), cephalothin practiced; the animals were allowed to graze during the (30 mcg), ampicillin (25 mcg), norfloxacin (10 mcg), day for 4-8 h and housed in an enclosed shed during oxacillin (1 mcg), nitrofurantoin (100 mcg) and night. Cases with high fever, anorexia, anemia, and tetracycline (30 mcg). The diameter of the zone of palpable enlargements of one or more of the superficial inhibition was measured and results were interpreted lymphnodes were observed. Tentatively, these cases as per the zone of inhibition size chart supplied by the were diagnosed as that of CLA. The pus samples and aspirates of lymphnodes were collected in nutrient RESULTS AND DISCUSSION
broth for bacterial isolation.
Bacteriological investigations: Primary isolation
The goat farm had a total strength of 70 animals was attempted on sheep blood agar plates by streaking and during the year 2010, 12 cases of CLA were a drop of nutrient broth with bacterial growth. The observed. The most common clinical findings in theaffected animals were enlargement of superficial lymphnodes i.e. mandibular, prescapular, prefemoral and supramammary lymphnodes. The enlarged makes it difficult to control and prevent the disease lymphnodes ruptured and exposed tough fibrous capsule fully, which in turn leads to considerable economic discharging thick greenish pus. In addition to lymphnode losses to the farmers (Baird and Fontaine, 2007).
enlargement, the affected animals had high fever, Control of the spread of CLA is of utmost importance anorexia, emaciation, pneumonia which was followed to ensure healthy marketable animals. To control and by death in severe cases. The clinical signs like prevent the disease, various types of approaches can pneumonia, arthritis and mastitis were also observed in be made like medicinal/surgical treatment, culling and a few animals in this study. Ghanbarpour and vaccination etc. In this study, we drained the pus after Khaleghiyan (2005) and Sharpe et al. (2010) also giving an incision at lower part of the abscess area with reported the cases of CLA in goats with more or less the care that the pus material from infected lymph nodes/abscess area was collected and disposed it off The organisms were isolated on inoculation of as biologic waste. The pus material was not allowed pus material on blood agar plates. Medium sized, to drain into bedding or soil. The affected animals were creamy white colonies appeared on incubation of these also treated with antimicrobials based on sensitivity plates at 370C for 24 h. These colonies were surrounded pattern along with supportive therapy in recommended by a narrow zone of hemolysis following 48 h of incubation. Gram staining of smears showed Gram REFERENCES
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