HIGHLY INDUCIBLE TET-ON VECTOR SYSTEM Summary of the Offer
developed. The original tetR do not contain
Public Health System (SSPA) has developed
a TetR(Tetracycline repressor)-based al -in-
allow the design of a less-toxic Tet-inducible
Description of the Offer
expression cassette that maintain the endogenous characteristics of the regulated-
Inducible gene expression systems based
promoters and do not transactivate other
research tools and are constantly developed
and are reproducible only if the doxycycline-
clinical application. Among the existing
inducible transcriptional gene regulatory
cloning or antibiotic selection. One of the
reasons for this requirement is the high
concentrations of TetR required to block
system is the most widely exploited tool for
controlling gene expression. However, al
these tetracycline-inducible system require
There are more autoregulatable lentiviral
a tetracycline-dependant-transactivator to activate the regulated promoter. The
vectors without the requirement of antibiotic
transcriptional activity has several undesired
several potential drawbacks that could limit
based al -in–one lentiviral system that
efficiently generates doxycycline-responsive
cloning and/or antibiotic selection. This
vector called CEST, is the result of the
homology to the TetO operon) sites can activate cellular genes.
combination of all the elements required for
Tet-ON regulation into a single lentiviral
responsive cassette (CMV-TetO) driving the
expression of the transgene and the SFFV
protein. This vector efficiently produced
primary cell lines such as 293T and human
infection) with the CEST vector and that
Benefits of the Offer IPR-Data
The regulatable gene expression system has
The technology is protected by a european
numerous advantages properties that make
it particularly suitable for application to gene
therapy and basic research. For example,
What are we looking for?
the system provides an “on”/”off” switch
for gene expression that allows for regulated
This research group is looking to establish a
dossing of a gene product in cell lines or in
a subject. This vector efficiently generates
Key words
doxycycline-responsive derivates include:
Category: Stem Cel s; Laboratory Materials
immortalized human fibroblasts. Hematopoietic stem cells and embryonic stem cells doxycycline-responsive are very hard to obtain with the CEST. The system is highly responsive to low doses of doxycycline with 1 ng/ml been sufficient to reach peak expression in cells containing up to 4 copies per genome. CEST vector do not cause toxic effects. The research group have analyzed the potential alteration of TetR overexpression in hMSC, an important target for cel -gene therapy applications. They did not found any alteration on the phenotype or in the cel cycle status on hMSCs that were transduced at high MOI (multiplicity of
J. vet. Pharmacol. Therap. doi: 10.1111/j.1365-2885.2009.01056.xIn vitro kinetics of hepatic albendazole sulfoxidation in channel catfish(Ictalurus punctatus), tilapia (Oreochromis sp.), rainbow trout (Oncorhynchusmykiss) and induction of EROD activity in ABZ-dosed channel catfishGonza´lez, J. F., Shaikh, B., Reimschuessel, R., Kane, A. S. In vitro kinetics ofhepatic albendazole sulfoxidation
ACTA_SOCIOLOGICA.qxd 2012.06.22. 14:43 Page 29 KISS ZSUZSANNA Hagyományos és modern kulturális határok Zala megye nyilvános élete az 1860-as években Elõadásomban Zala megye nyilvános életének egy rövid korszakát fogom megvizs-gálni: az 1848/49-es forradalmat követõ idõszaknak, a neoabszolutizmusnak a má-sodik felét, az 1860-as éveket. Ezt a korszakot a történetír