Laminarinase.narod.ru

Chemistry of Natural Compounds, Vol. 45, No. 1, 2009 POLYSACCHARIDES AND STEROLS FROM GREEN ALGAE
Caulerpa lentillifera AND C. sertularioides
N. M. Shevchenko,1 Yu. V. Burtseva,1 T. N. Zvyagintseva,1* T. N. Makar′eva,1
O. S. Sergeeva,1 A. M. Zakharenko,1 V. V. Isakov,1 Nguyen Thi Linh,2
Nguyen Xuan Hoa,2 Bui Minh Ly,3 and Pham Van Huyen3
Sterols and polysaccharides of green alga Caulerpa lentillifera grown under laboratory conditions and inmariculture and polysaccharides of green alga C. sertularioides grown under natural conditions werestudied. The sterol fraction consisted of C27-C29 steroidal alcohols with Δ5-unsaturation in the steroid coreregardless of the growth conditions. The dominant (79.9%) steroid component of the sterol fraction wasclionasterol. The water-soluble fraction of C. lentillifera grown under laboratory conditions was a mixtureof 1,4-α- and 1,3-β-D-glucans and protein. The same fraction isolated from C. lentillifera grown inmariculture contained only protein. The water-soluble fraction of C. sertularioides grown under naturalconditions contained 1,3;1,6-β-D-galactan sulfated at C2. The principal components of the base-solublepolysaccharide fractions from all algae samples were 1,4-α-D-glucans. Key words: green algae, Caulerpa lentillifera, C. sertularioides, polysaccharides, sterols, clionasterol.
Marine algae, including representatives of the green algae (Chlorophyta), to which the genus Caulerpa belongs, are a rich source of sterols that differ in chemical structure from cholesterol, the main sterol of higher animals. Steroids areimportant biologically as hormones, vitamins, and structural components of biomembranes.
Polysaccharides are an important component of algae. The heightened interest in them is related to their broad spectrum of biological activity. For example, polysaccharides exhibiting anticoagulant, antitumor, and other activities have beenisolated from green algae Caulerpa racemosa, C. brachypus, C. okamurai, C. scapelliformis, Chaetomorpha crassa, C. spiralis,Codium adhaerens, C. fragille, C. latum, Enteromorpha compressa, Monostroma nitidum, and Ulva spp. [1-4].
The goal of our work was to study polysaccharides and sterols from the green algae Caulerpa lentillifera and C. sertularioides, the composition and structure of which have not been previously studied.
We used samples of C. lentillifera grown under laboratory conditions at 25-30°C (natural illumination, collected January 20, 2005, sample 1) and in man-made aquifers in the shade (collected April 15, 2007, Khanh Hoa Province, Ninh HoaDistrict, Viet Nam, sample 2) and also samples of C. sertularioides collected under natural conditions in May 2007(sample 3).
Steroid components of C. lentillifera were isolated from samples 1 and 2 (see Experimental) and identified by mass spectra of their acetates and their GC behavior over a capillary column. Table 1 lists the composition of the sterol fraction ofsample 1. The composition and amount of sterols isolated from different samples were identical.
Table 1 shows that the sterol fraction consists of C27-C29 steroidal alcohols with Δ5-unsaturation in the steroidal core.
The dominant (79.9%) sterol was clionasterol, for which the absolute configuration at C-24 was determined by comparing PMRspectra with model 24S and 24R C29 sterols [5, 6]. The PMR spectrum contained resonances characteristic of the 24Sconfiguration at 0.68 (3H, s, CH3-18), 1.01 (3H, s, CH3-19), 0.93 (3H, d, J = 6.8, CH3-21), 0.83 (3H, d, J = 6.8, CH3-26), 0.81(3H, d, J = 6.8, CH3-27), 0.86 (3H, t, J = 7.0, CH3-29), 3.5 (m, 1H, H-3), 5.6 (m, 1H, H-6).
1) Pacific Institute of Bioorganic Chemistry, Far-East Branch, Russian Academy of Sciences, 690022, Vladivostok, pros. 100-Letiya Vladivostoka, 159, Russia, fax: +7(4232) 31 40 50, e-mail: zvyag@piboc.dvo.ru; 2) Institute ofOceanography, Vietnamese Academy of Science and Technology, Vietnam, fax: +84(58) 59 00 34, e-mail:haiduong@dng.vnn.vn; 3) Nhatrang Institute of Technology Research and Application, Vietnamese Academy of Science andTechnology, Vietnam, fax: +84(58) 52 18 47, e-mail: bminhly@dng.vnn.vn. Translated from Khimiya Prirodnykh Soedinenii,No. 1, pp. 5-8, January-February, 2009. Original article submitted July 17, 2008.
0009-3130/09/4501-0001 2009 Springer Science+Business Media, Inc.
TABLE 1. Sterol Fraction of Alga Caulerpa lentillifera Retention time (relative to cholesterol acetate) 24-Methylcholesta-5,22E-dien-3β-ol 24-Ethylcholesta-5,22E-dien-3β-ol 24S-Ethylcholest-5-en-3β-ol (clionasterol) The composition of the sterol fraction was similar to sterols of other algae of the genus Caulerpa that were studied previously. The main sterol of this genus was practically always clionasterol [7].
Three polysaccharide fractions, P1, water-soluble, and P2 and P3, base-soluble, were isolated and characterized from Results for compounds of water- (P1-1) and base-soluble (P2-1 and P3-1) fractions from sample 1 are listed below.
The protein content in fraction P1-1 was rather high, about 20%. The amino-acid composition of the protein components offraction P1-1 (amino-acid content expressed in mole percent) was as follows: Asp (13.3), Thr (6.7), Ser (7.2), Glu (18.2), Gly(16.0), Ala (9.1), glucosamine (1.2), Val (5.2), Ile (3.2), Leu (4.3), Tyr (0.9), Phe (1.5), His (0.5), Lys (3.7), Arg (1.6), citrulline(3.5), Pro (3.9). The protein was dominated by Glu, Asp, Gly, and Ala. Proline (3.9) and its precursor citrulline (3.5) areconstant components of plant cells [8] and were also present in appreciable amounts.
Fractions were purified of protein by the Sevag method. The ranges of molecular weights of fraction P1-1 and P2-1 polysaccharides that were determined by gel chromatography were 20-60 and 20-40 kDa, respectively. Fraction P3-1 consistedof higher molecular weight polysaccharides with MW >70 kDa. A study of the monosaccharide composition showed that allfractions of sample 1 consisted of Glc, Gal, Man, and Xyl in different proportions (Table 2). Glucose dominated themonosaccharide composition of all these fractions.
The 13C NMR spectrum of fraction P1-1 contained resonances characteristic of 1,4-α-D-glucan (amylose) with C1 (101.21 ppm), C2 (72.96), C3 (74.91), C4 (78.99), C5 (72.96), C6 (62.29), and 1,3-β-D-glucan (laminaran) with C1(104.08 ppm), C2 (74.9), C3 (86.1), C4 (69.85), C5 (77.3), C6 (62.47) [9]. 1,3-β-D-Glucan has been observed previously onlyin Caulerpa simpliciuscula [10]. Resonances characteristic of 1,4-α-D-glucan dominated the 13C NMR spectra of fractions P2-1and P3-1.
IR spectra of water- and base-soluble polysaccharide fractions isolated from C. lentillifera (sample 1) lacked absorbances at 1230-1255 cm−1 and 815-850, which are typical of sulfate groups (spectra are not shown). For example,polysaccharides containing sulfates have been isolated from Ulva rigida, Spongomorpha indica, C. racemosa, and C. dwarkense[1, 11, 12].
Water-soluble fraction P1-2 was isolated from C. lentillifera grown in mariculture (sample 2, yield about 2% of the defatted alga weight) and consisted of practically only protein. The sugar content according to the phenol—H2SO4 method wasless than 0.1% of the fraction weight. Monosaccharides also were not found in this fraction after hydrolysis. Base-solublefractions P2-2 and P3-2, like sample 1, contained amylose according to 13C NMR spectroscopy.
Three fractions P1-3, P2-3, and P3-3 were also isolated and characterized from wild green alga C. sertularioides (sample 3). Analysis of the monosaccharide composition of fraction P1-3 showed predominantly galactose and to a lesser extentglucose (Table 2). The 13C NMR spectrum of fraction P1-3 showed strong resonances with chemical shifts 102.6, 79.3, 77.1,74.6, 70.4, and 68.7 ppm. Furthermore, weaker resonances corresponding to 1,4-α-D-glucan were also present. The 13C NMRspectra suggested that fraction P1-3 contained acetyls (21.71 ppm CH3; 175.92 ppm C=O).
TABLE 2. Properties of Polysaccharides from Fractions P1, P2, and P3 from Green Algae Caulerpa lentillifera andC. sertularioides Monosaccharide composition, mole fractions ______*Yield determined as percent of defatted alga dry weight.
Deacetylation, despite the loss of information about the position of the acetyls, is often used to make NMR spectra of polysaccharides simpler and easier to interpret [13]. Deacetylation of fraction P1-3 followed by dialysis on a centrifugalmembrane separated a low-molecular-weight 1,4-α-D-glucan and produced fraction P1(1)-3 in 44% yield that contained apolysaccharide consisting mainly of galactose. The IR spectrum of fraction P1(1)-3 exhibited absorption bands at 1252 cm−1(S=O stretch) and at 818 (C–O–S bending) that are characteristic of sulfates that could be located on either C2 or C6 of galactose(spectra not shown) [14]. Resonances in the 13C NMR spectrum of this polysaccharide were assigned using COSY and HSQC2D correlation spectra as 102.6 ppm (C1), 79.3 (C2), 77.1 (C3), 68.7 (C4), 74.6 (C5), and 70.4 (C6) [9]. The anomeric protonHa bound to C1 resonated at 4.63 ppm with SSCC J = 7.3 Hz; Hb, at δ 4.38 ppm. This was indicative of the β-configurationof the glucoside bond with C2 substituted by SO3. The chemical shift of C6 of 70.6 ppm was consistent with glycosylation ofthe CH2OH of the β-glycoside bond. This was confirmed by 13C NMR data reported for sulfated 1,3;1,6-β-D-galactans [15].
Thus, the studied polysaccharide was a 1,3;1,6-β-D-galactan sulfated at the galactose C2 atom.
Resonances typical of a 1,4-α-D-glucan dominated 13C NMR spectra of fractions P2-3 and P3-3. Fraction P2-3 contained a small impurity of 1,3;1,6-β-D-galactan sulfated at the galactose C2, which was the main component of fraction P1-3.
Thus, the sterol composition of C. lentillifera, regardless of the cultivation conditions, consisted mainly of clionasterol.
According to the literature, this is typical for algae of the genus Caulerpa. The polysaccharide composition of the studiedspecies grown in mariculture lacked sulfated polysaccharides. Sulfated 1,3;1,6-β-D-galactan was observed only in wildC. sertularioides. A small amount of 1,3-β-D-glucan, the main reserve polysaccharide of brown and diatomaceous algae [16],was observed in C. lentillifera grown under laboratory conditions. C. lentillifera is rich in water-soluble proteins, the amino-acid composition of which is rather variable.
EXPERIMENTAL
Isolation of Sterol Fraction. Moist alga (3.0 g dried alga) was extracted with ethanol at room temperature
(3 × 200 mL). The ethanol extract was evaporated to dryness in vacuo (500 mg), dissolved in water, and extracted with butanol(3 × 50 mL). The butanol extracts were combined, evaporated to dryness (100 mg), and chromatographed twice over a silicagel column using CHCl3 to produce the sterol fraction (9 mg, 0.3% of dry alga weight) that was pure by TLC.
Polysaccharide Extraction [14]. Green alga C. lentillifera that was defatted with ethanol and ground (samples 1 and
2, 3.2 and 12.8 g, respectively) and C. sertularioides (sample 3, 12.0 g) were extracted with water (200, 900, and 800 mL,respectively) at 80°C for 30 min. The procedure was repeated three times. The total extract was lyophilized after evaporation,dialyzed, and precipitated with ethanol (1:4) to afford water-soluble fractions (P1) from the three alga samples P1-1 (87 mg),P1-2 (38 mg), and P1-3 (120 mg), respectively.
The remaining alga was extracted with KOH solution (1 M) containing NaBH4 (20 mM) at 37°C (4 h) and then at 4°C for 17 h. The resulting extract was neutralized with AcOH (until the pH was 6.0), dialyzed by ultrafiltration usingMillipore-1 kDa membranes (Millipore, USA). Base-soluble fractions (P2) from the three alga samples, P2-1 (130 mg), P2-2(78 mg), and P2-3 (99 mg) were lyophilized.
The remaining alga was treated with KOH solution (4 M) containing NaBH4 (20 mM) and extracted analogously. The yields of base-soluble fractions (P3) from the three alga samples were P3-1 (113 mg), P3-2 (315 mg), and P3-3 (272 mg),respectively.
Purification from protein of fraction P1-1 (87 mg in 2 mL) was carried out by the Sevag method [17].
Deacetylation of polysaccharides of fraction P1-3 (100 mg) was carried out by treatment with aqueous ammonia
solution (12%) for 14 h at 37°C. The resulting precipitate was removed by centrifugation. The supernatant was dialyzed byultrafiltration using Millipore-10 kDa centrifugal membranes (Millipore, USA) to afford two fractions, fraction P1(1)-3 abovethe membrane (44 mg) and fraction P1(2)-3 under the membrane (21 mg).
Molecular weights of polysaccharides were estimated by gel-filtration over a Superdex 75-HR 10/30 column
(1.0 cm × 30 cm) (Amersham Pharmacia Biotech AB, USA) equilibrated with phosphate buffer (pH 7.2) containing NaCl(0.15 M) and eluted by the same buffer at 0.4 mL/min. The standards were dextrans with molecular weights 10, 20, 40, and80 kDa.
Neutral sugars were determined by the phenol-H2SO4 method [18].
Protein content was determined by the Bradford method [19].
Amino-acid composition of proteins in water-soluble fraction P1-1 were determined after hydrolysis of the fraction
by HCl (6 M) at 100°C (24 h) in a Biochrom-30 analyzer (200 × 4.6, Ultropac-8 μm [Li+]). The ratio of amino acids wasdetermined by comparing peak areas of the samples with those of a standard mixture of amino acids (Sigma, USA).
Monosaccharide Composition of Polysaccharides. Fractions of polysaccharides (5 mg each) were hydrolyzed by
TFA (2 M) at 100°C (4 h). The monosaccharide compositions of the fractions were determined on an IC-5000 Biotronikcarbohydrate analyzer (Germany, Shim-pack ISA-07/S2504 column, 0.4 × 25 cm) using potassium borate buffer at elution rate0.6 mL/min. Sugars were detected using the bicinchonate method with a Shimadzu C-R2 AX integration system.
Monosaccharides (Rha, Rib, Man, Fuc, Gal, Xyl, Glc) were used as standards.
IR spectra of polysaccharides in films were recorded on a Vector 22 Fourier transform FTIR spectrophotometer
(Bruker, Germany) at 4 cm−1 resolution.
GC of sterol fraction was performed in an Agilent 6850 chromatograph with an HP-5MS capillary column
(30 m × 0.25 mm, Agilent, USA) at 290°C.
GC—MS Analysis. Sterol fractions were acetylated by Ac2O—Py (1:1) for 16 h at room temperature and analyzed
in an Hewlett—Packard HP 6890 mass spectrometer (ionization energy 70 eV, He carrier gas) with an HP-5MS capillary column(30 m × 0.25 mm, Agilent, USA) at 270°C.
Preparative column chromatography of sterol fractions was carried out over KSK silica gel (50-160 μm, Sorbpolimer,
TLC of sterol fractions was performed on Sorbfil plates with a fixed layer of STKh-1A silica gel (5-17 μm,
13C NMR spectra of polysaccharides were recorded on a Bruker Avance DPX-300 spectrometer (D2O, operating
frequency 75.5 MHz, methanol internal standard, δC 50.15 ppm at 60°C).
PMR spectra of sterols were recorded on a Bruker DRX 500 spectrometer (CDCl3, operating frequency 123.8 MHz,
ACKNOWLEDGMENT
The work was supported financially by grants of the RFBR 07-04-90010-viet-a, FEB RAS, and RAS Presidium Program "Cellular and Molecular Biology." REFERENCES
P. Ghosh, U. Adhikari, P. Ghosal, C. Pujol, M. Carlucci, E. Damonte, and B. Ray, Phytochemistry, 65, 3151 (2004).
J.-B. Lee, K. Hayashi, M. Maeda, and T. Hayashi, Planta Med., 70, 813 (2004).
M. Maeda, T. Uehara, N. Harada, M. Sekiguchi, and A. Hiraoka, Phytochemistry, 30, 3611 (1991).
N. Noda, H. Amano, K. Arashime, and K. Nisizwa, Hydrobilogia, 204/205, 577 (1990).
I. Rubinstein, L. J. Goad, A. D. H. Clague, and L. J. Mulheirn, Phytochemistry, 15, 195 (1976).
T. N. Makarieva, I. A. Bondarenko, A. S. Dmitrenok, V. M. Boguslavsky, V. A. Stonik, V. I. Chernih,
and S. M. Efremova, J. Nat. Prod., 54, 953 (1991).
L. DeNapoli, S. Magno, L. Mayol, and E. Novellino, Phytochemistry, 21, 1993 (1982).
N. I. Shevyakova, Fiziol. Rast., 3, 768 (1983).
T. N. Zvyagintseva, N. I. Shirokova, and L. A. Elyakova, Bioorg. Khim., 20, 1349 (1994).
R. J. Howard, S. W. Wright, and B. R. Grant, Plant Physiol., 58, 459 (1976).
A. K. Siddhanta, M. Shanmugam, K. H. Mody, A. M. Goswami, and B. K. Ramavat, Int. J. Biol. Macromol., 26, 151
(1999).
E. V. Rao, N. V. Rao, and K. S. Ramana, Phytochemistry, 30, 1183 (1991).
M. I. Bilan, A. A. Grachev, N. E. Ustuzhanina, A. S. Shashkov, N. E. Nifantiev, and A. I. Usov, Carbohydr. Res., 337,
719 (2002).
K. Chattopadhyay, U. Adhikari, P. Lerouge, and B. Ray, Carbohydr. Polym., 68, 407 (2007).
M. I. Bilan, E. V. Vinogradova, A. S. Shashkov, and A. I. Usov, Carbohydr. Res., 342, 586 (2007).
T. J. Painter, Pure Appl. Chem., 55, 677 (1983).
A. M. Shtaub, in: Methods of Carbohydrate Chemistry [Russian translation], N. K. Kochetkov, ed., Mir, Moscow(1967), 261.
M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Robers, and F. Smith, Anal. Chem., 28, 350 (1956).
M. Bradford, Anal. Biochem., 72, 248 (1976).

Source: http://www.laminarinase.narod.ru/09_45_1.pdf