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Layout284jumonline.qxp:Layout 1 3/17/09 2:30 PM Page 541 Case Report
Inspissated Bile Syndrome in a
Neonate Treated With Cefotaxime
Sonographic Aid to Diagnosis, Management,and Follow-up Tamir Miloh, MD, Henrietta Kotlus Rosenberg, MD,Israel Kochin, MD, Nanda Kerkar, MD holelithiasis in neonates is usually pigmentary and composed of calciumbilirubinate.1,2 In children, cholelithisasis and inspissated bile or sludge areassociated with prematurity, cystic fibrosis, hemolytic diseases, hemorrhage, Ccongenital heart disease, starvation, total parenteral nutrition, infection, dehydration, ileal resection, abdominal surgery, congenital anomalies of the biliarytract, chronic liver disease, medications (ceftriaxone), and up to 43% of cases are idio-pathic.2,3 We report the case of a neonate who had recurrent cholestasis after beingtreated for sepsis with cefotaxime. Case Report
This 5-lb 15-oz full-term female neonate had an uncom- ALT, alanine aminotransferase; AST, aspartate amino- plicated perinatal history, a normal vaginal delivery, and transferase; CBD, common bile duct; GGT, γ-glutamyltransferase; UDCA, ursodeoxycholic acid normal newborn screening results. She was brought to alocal hospital at 2 weeks of age with fever, abdominal dis-tension, vomiting, jaundice, and acholic stools. Pertinentlaboratory results included normal electrolyte levels, an aspartate aminotransferase (AST) level of 131 IU/L, analanine aminotransferase (ALT) level of 41 IU/L, and an alkaline phosphatase level of 106 IU/L (all normal for Received October 30, 2008, from the Departments age). Abnormal laboratory values included a γ-glutamyl- of Pediatrics (T.M., H.K.R., I.K.) and Radiology transferase (GGT) level of 613 IU/L (normal value for age, (H.K.R., N.K.), Mount Sinai Medical Center, NewYork, New York USA. Revision requested <300 IU/L), total/direct bilirubin levels of 4.5/2.9 mg/dL November 19, 2008. Revised manuscript accepted (normal, <1.2/0.8 mg/dL), and a blood culture positive Address correspondence to Tamir Miloh, MD, for Aeromonas hydrophila and Klebsiella pneumoniae.
Department of Pediatrics, Mount Sinai Medical Initial sonography showed ascites with an unremarkable- Center, 1 Gustave L. Levy Pl, New York, NY 10029 appearing liver and no evidence of intrahepatic or extra- E-mail: firstname.lastname@example.org hepatic biliary ductal ectasia or biliary sludge. 2009 by the American Institute of Ultrasound in Medicine • J Ultrasound Med 2009; 28:541–544 • 0278-4297/09/$3.50 284jumonline.qxp:Layout 1 3/17/09 2:30 PM Page 542 Inspissated Bile Syndrome in a Neonate Treated With Cefotaxime
The neonate was treated with intravenous ampi- bin levels of 4.4/4.3 mg/dL. Sonography showed cillin and cefotaxime for 10 days. Enteral feedings a cholestatic pattern in the liver, dilatation of the were resumed by the third hospital day. On dis- intrahepatic and extrahepatic biliary ducts, charge, the jaundice had resolved (bilirubin, common bile duct (CBD) size of 5 mm, a fluid- 1/0.5mg/dL), and the liver enzymes declined to an debris level in the extrahepatic biliary ducts AST level of 38 IU/L, an ALT level of 18 IU/L, and consistent with dependent sludge, and a large sludge ball in the gallbladder, which contained One week after discharge, jaundice and acholic nonshadowing brightly echoic foci suspicious for stools recurred without any associated symp- cholelithiasis (Figure 1). She was given ursodeoxy- toms, and she was referred to our institution.
cholic acid (UDCA) at a dose of 15 mg/kg twice a Physical examination was unremarkable aside day, fat-soluble vitamins, a medium-chain triglyc- from mild hepatomegaly. Laboratory blood tests eride-rich formula, and intravenous hydration.
revealed an AST level of 101 IU/L, an ALT level of Her stools became pigmented after 2 days, and 33 IU/L, an alkaline phosphatase level of 250 subsequent abdominal sonography on the sixth IU/L, a GGT level of 1197 IU/L, and total biliru- Figure 1. A, Sonogram of the dilated common hepatic duct (large arrow) and dilated CBD (small arrows) with a fluid-debris level
noted within the distal CBD due to biliary sludge. The diameter of the CBD measured 0.5 cm. B, Color Doppler sonogram clearly dif-
ferentiating between the dilated extrahepatic ducts and the surrounding vascular structures. Once again, sludge is noted within the
distal CBD (arrow). C, Transverse sonogram in the region of the head of the pancreas showing brightly echoic sludge (arrow) filling
the distal CBD at the level of the head of the pancreas. GB indicates gallbladder. D, Longitudinal sonogram of the gallbladder show-
ing the presence of a large sludge ball (arrow) within the lumen, which contains several small focal nonshadowing more brightly
echoic foci suspicious for precipitated material. The gallbladder is otherwise sonographically normal.
284jumonline.qxp:Layout 1 3/17/09 2:30 PM Page 543 Miloh et al
reduction in the size of the CBD to 3 mm. She nosis of anatomic abnormalities that may cause was discharged and continued to receive UDCA.
biliary obstruction and in most cases obviates At 6 months of age, she was thriving; bilirubin the need for higher-tech imaging studies and levels were 0.2/0.1 mg/dL; transaminase levels prevents unnecessary interventional procedures were less than 50 IU/ml; and her GGT level was 23 IU/L (normal). Delayed sonography showed The incidence of inspissated bile syndrome is 1 complete resolution of the biliary ductal dilata- per 175,000 live births in England and accounts for about 8% of all surgical jaundice duringinfancy.5 Biliary sludge appears sonographically Discussion
as low-level echoes. On microscopy a mixture ofparticulate matter appears when various biliary Early diagnosis of the underlying etiology of solutes precipitate cholesterol, calcium bilirubi- cholestasis in neonates is essential so that appro- nate or other calcium salts, mucus, undefined priate therapy can be promptly instituted. A residues, and protein-lipid complexes. The diag- direct bilirubin level of greater than 1 mg/dL (if nosis of sludge is almost always based on imaging.
the total bilirubin level is <5 mg/dL) or greater The pathogenesis of sludge is similar to that of than 20% of the total bilirubin level (if the total gallstones, which are formed from precipitating bilirubin is level is >5 mg/dL) is diagnostic of sludge.6 There are many predisposing factors to conjugated hyperbilirubinemia. Jaundice, per- the development of inspissated bile, sludge, or sistent acholic stools, and an elevated GGT level cholelithiasis in neonates.1–3 Ceftriaxone pseudo - are suggestive of obstructive jaundice. In the lithiasis, composed of precipitated ceftriaxone, is neonatal period, the differential diagnosis of reported to occur in 29.5% to 45.7% of children obstructive cholestasis includes biliary atresia, a treated with ceftriaxone.3 The pseudolithiasis choledochal cyst, gallstones or biliary sludge, occurs after 4 to 22 days (mean, 9 days) of ceftriax- inspissated bile syndrome, cystic fibrosis, neona- one therapy and resolves after 2 to 63 days (mean, tal sclerosing cholangitis, and congenital hepatic 15 days) from the end of treatment.7 Cefotaxime, a fibrosis/Caroli disease.4 Meticulous sonographic third-generation cephalosporin, was previously evaluation of the liver, spleen, pancreas, biliary reported to be associated with pseudolithiasis in 2 ducts, and biliary vessels along with clinical and of 34 infants (6%) who had cholelithisis.1 This laboratory correlation allows for accurate diag- relationship was not found in 38 older childrenreceiving cefotaxime for 4 to 7 days.3 Therefore,this association may be unique in the neonatalpopulation.
Figure 2. After treatment with UDCA, fat-soluble vitamins, and
intravenous hydration, the neonate’s stools became pigmented
In most patients, removal of the precipitating after 2 days, and subsequent abdominal sonography showed no factor can lead to spontaneous resolution of bil- evidence of intrahepatic or extrahepatic biliary ductal ectasia, iary sludge. The refractory cases of inspissated stones, sludge, or pericholecystic fluid. bile syndrome and those associated with biliary-type pain, cholecystitis, cholangitis, or pancre-atitis are treated with open or laparoscopiccholecystectomy. Some may require endo-scopic retrograde cholangiopancreatographicsphincterotomy, percutaneous transhepaticcholangiographic saline flushing, or infusion ofN-acetylcysteine into the extrahepatic biliaryducts to prevent further episodes of cholangitisand pancreatitis.2 In asymptomatic patients, thesludge can be managed expectantly. Our patient’scholestasis resolved with hydration and high-dose (30-mg/kg/d) UDCA. Ursodeoxycholic acidis a hydrophilic bile acid that enriches the bile 284jumonline.qxp:Layout 1 3/17/09 2:30 PM Page 544 Inspissated Bile Syndrome in a Neonate Treated With Cefotaxime
acid pool, decreases the biliary saturation of Keizman D, Ish-Shalom M, Konikoff FM. The clinical signif- cholesterol, and may prevent sludge formation.8,9 icance of bile duct sludge: is it different from bile ductstones? Surg Endosc 2007; 21:769–773.
It was found to resolve symptoms in pediatricpatients with gallstones; however, the stones Herek O, Pakdemirli E, Kocer N. Ceftriaxone-associated bil-iary pseudolithiasis in children. Eur Radiol 2001; 11:902.
transiently disappeared in only 2 of 15 patients inone study10 and in 8 of 180 in another.11 Jain R. Biliary sludge: when should it not be ignored? CurrTreat Options Gastroenterol 2004; 7:105–109.
The neonate we report was born full term, had normal newborn screening results, and was not Arslanoglu S, Moro GE, Tauschel HD, Boehm G.
Ursodeoxycholic acid treatment in preterm infants: a pilot dehydrated when she had inspissated bile syn- study for the prevention of cholestasis associated with total drome. The initial outside liver sonography dur- parenteral nutrition. J Pediatr Gastroenterol Nutr 2008; ing the septic period did not reveal sludge despite biochemical cholestasis. Obstructive Gamba PG, Zancan L, Midrio P, et al. Is there a place for jaundice recurred 1 week after recovery from medical treatment in children with gallstones? J Pediatr sepsis. Because the neonate did not have any other risk factors for development of inspissated Della Corte C, Falchetti D, Nebbia G, et al. Management of bile, the potential association was treatment with cholelithiasis in Italian children: a national multicenterstudy. World J Gastroenterol 2008; 14:1383–1388.
cefotaxime, which may be unique in the neona-tal period. In conclusion, obstructive jaundice in neonates, as a consequence of inspissated bile or sludge,may follow treatment with cefotaxime and is like-ly to respond to hydration and medical manage-ment with UDCA. We emphasize the use ofsonography in the diagnosis, treatment, and fol-low-up of neonates with inspissated bile syn-drome, which in most cases obviates the need forhigher-tech imaging studies and prevents unnec-essary interventional procedures or surgery. The cholestasis and extrahepatic obstructionresolved with hydration and high-dose UDCA.
Klar A, Branski D, Akerman Y, et al. Sludge ball, pseu-dolithiasis, cholelithiasis and choledocholithiasis fromintrauterine life to 2 years: a 13-year follow-up. J PediatrGastroenterol Nutr 2005; 40:477–480.
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Moyer V, Freese DK, Whitington PF, et al. Guideline for theevaluation of cholestatic jaundice in infants: recommenda-tions of the North American Society for PediatricGastroenterology, Hepatology and Nutrition. J PediatrGastroenterol Nutr 2004; 39:115–128.
Gunnarsdóttir A, Holmqvist P, Arnbjornsson E, KullendorffCM. Laparoscopic aided cholecystostomy as a treatment ofinspissated bile syndrome. J Pediatr Surg 2008; 43:e33–e35.
Rang & Dale's Pharmacology Table Of Contents: General principles How drugs act: cellular aspects---excitation, contraction and secretion Chemical mediators Chemical mediators and the autonomic nervous system Other peripheral mediators: 5-hydroxytryptamine and purines Local hormones, inflammation and immune reactions Anti-inflammatory and immunosuppres ant drugs Drugs a